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Reference gene normalization is used to control for sources of pre-analytical and analytical variability as well as allow for variable RNA inputs.
In a laboratory setting, we can control preanalytical and analytical variability, leaving the biological component as the only true variable.
The aim of this study was to evaluate the analytical variability of the FT proteins.
The analytical variability of the Fibrotest (FT) parameters raises the issue of the test's reliability for routine use.
Achieving an appropriate consideration of the analytical variability in assay procedures and setting acceptance criteria for analytical validations is however much more difficult than usually described.
Analytical variability is an important consideration.
For reference, average analytical variability was 3.4% from five sweeps during analysis.
All extractions were performed in duplicate to account for analytical variability.
All reactions for both archaeal and bacterial amoA qPCR runs were carried out in triplicate to account for analytical variability.
The typical analytical variability as established during method validation should be considered in calculating the release limit. .
The typical analytical variability as established during method validation should be considered in calculating the release limit.
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