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(b) Analytical size exclusion HPLC.
Analytical size exclusion chromatography shows that SopDΔN is monomeric and probably globular in aqueous solution.
Purity was assessed by Coomassie staining after sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and analytical size exclusion high-pressure liquid chromatography (HPLC) (BioSep SEC-s2000, Phenomenex, Cheshire, UK).
Prod1 purified as a monomer as assessed by analytical size exclusion chromatography.
The chromatogram obtained during the final step of purification using analytical size exclusion shows only one peak (Figure 2).
The size of NerA in solution was determined by analytical size exclusion chromatography.
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Protein purity and monodispersity were tested by SDS-PAGE and analytical size-exclusion chromatography (aSEC).
By neglecting the elastic strain increments in the plastic zone, approximate analytical size-dependent solutions are also derived.
The hydrodynamic and self-associative properties of a series of PaDDAH interface mutants were analyzed by concentration-dependent analytical size-exclusion chromatography and sedimentation equilibrium analytical ultracentrifugation.
Analytical size-exclusion radio-HPLC traces were acquired using an Agilent 1200 Series HPLC system and a Phenomenex Biosep 2000 (300 × 7.8 mm) size-exclusion column with a phosphate-buffered saline mobile phase.
Purity was assessed by Coomassie staining after sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (SDS-PAGE; see Additional file 1) and analytical size-exclusion high-pressure liquid chromatography (HPLC) (BioSep SEC-s2000, Phenomenex, Cheshire, UK; see Additional file 1).
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