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Please see workflow diagram for overview on sample processing analytical endpoints (Figure 1).
These findings suggest that circadian rhythm should be considered in radiation research and that biological and analytical endpoints should be validated for circadian robustness.
Despite diurnal variation, main results on 131I-induced transcriptional regulation were reproduced irrespective of time of day, which validated the analytical endpoints used for radiogenomic biomarker discovery in vivo.
There were no significant differences between groups for the various diabetes-related analytical endpoints.
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Nevertheless, the consistent trend in signature gene regulation, meaning a general dominance of TH-associated sigenesre genes, was reproduced through the day, which validated this analytical endpoint with regard to circadian rhythm.
Thus, we advocate that circadian rhythm should be considered as an experimental variable in radiation research and that "circadian robustness" should be validated for the biological or analytical endpoint under investigation.
The analytical endpoint was defined as VAS 3+ tenderness of tennis elbow.
The analytical endpoint was defined as Visual Analog Score (VAS) 3+ tenderness of tennis elbow.
The main analytical endpoint (dependent variable) of the present report was psychological distress, as measured by the SCL-90-R.
The Global Severity Index (GSI) score, a measure of overall psychological distress, served as the primary analytical endpoint.
Although the GSI was used as our primary analytical endpoint, to enable comparisons with previous and future studies we present PST, PSDI and the 9 SCL-90-R dimensions scores in the supplemental material (Additional file 1: Table S1).
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