The analytic sensitivity of this system was assessed to be < 5 pg DNA per PCR reaction.
We therefore, compared the bias, accuracy and precision of FECRT results for Cornell-Wisconsin (analytic sensitivity = 1 egg per gram faeces (EPG)), FLOTAC (analytic sensitivity = 1 EPG) and McMaster method (analytic sensitivity = 10 EPG) across four levels of egg excretion (1 49 EPG; 50 149 EPG; 150 299 EPG; 300 600 EPG).
The key building blocks are: analytic sensitivity analysis, an adaptive finite element method, an accurate contact solver, and a sequential convex programing optimization algorithm.
However, it remains unclear whether FEC methods with an equal analytic sensitivity, but with different methodologies, result in equal FECRT results.
Finally, applying ultrasensitive chemiluminescence magnetic nanoparticles immunoassay (CL-MBs-nano-immunoassay) technology further increases the analytic sensitivity of the CLIA method.
The analytic sensitivity was determined by analyzing a half-logarithmic serial dilution of the IVT ranging from 100 to 0.1 copies/reaction.
Comparison of the results of available tests for CYP450 genotype with a reference standard suggests that the analytic sensitivity and specificity of available tests are generally high, although some serious concerns remain.
The analytic sensitivity (limit of detection (LoD)) is defined as the concentration (copies/reaction) of MERS-CoV specific RNA (IVT) molecules that can be detected with a positive rate of ≥95%%.
Since the aptamer concentration of 25 μM allowed full RT activity at 55 °C but significantly reduced RT activity at 31 °C, this concentration was chosen to survey the influence of the aptamer on the analytic sensitivity of the MERS-CoV assay.
Since most of the agents that RPM-TEI was designed to detect required BSL-3 or BSL-4 facilities for safe handling, they were substituted for analytic sensitivity testing by synthetic DNA fragments manufactured by BlueHeron Biotechnology, Inc., Bothell, WA (see supporting Table S3 for a complete list).
The assay had an analytic sensitivity of 4 nmol/l.
