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NM, AH, and JN conducted the majority of in vitro experimentation and data analysis, with a minor contribution from SMcF.
Scandura et al., (2008; 2011b) using 10 autosomal microsatellites, found the Sardinian WB clustering separately in a Bayesian analysis, with a minor overlap to other sampled populations.
In addition, the other genera were well grouped monophyletically in the analysis with a minor exception in the interrelationships of Kalligramma.
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The SNP data quality was then controlled by PLINK software [ 12] to exclude from further analysis SNPs with a minor allele frequency less than 0.01 and those that deviated from the Hardy Weinberg equilibrium (HWE) (P value < 1e−6).
The IL-22 SNPs selected for additional genotyping analysis were those with a minor allele frequency of >0.05 in the Han Chinese population and were located within the exons, 3′ and 5′ flanking regions and intron 1. Totally, 35 IL-22 SNPs were genotyped.
In addition, immunofluorescence analysis showed a minor population of cells with a cytoplasmic stain for SP. Taken together, these data indicate that MDDCs need to engulf and digest SP in order to stimulate the production of IL-12p70.
The SNPs selected for the analysis were non-synonymous with a minor allele frequency of at least 10%.
DNA samples were subjected to traditional L. monocytogenes MLST analysis [ 33, 34], with a minor modification to the annealing temperature for the bglA PCR (52°C not 45°C).
He received B.Eng. with a major in Engineering Structure Analysis at College of Engineering, and BEc with a minor in Economics at National School of Development from Peking University in 2014.
For the purpose of this analysis, only SNPs on autosomes with a minor allele frequency of greater than 0.2 and a missing value quota of not more than 20% were considered.
Further analysis was performed on variants with a minor allele frequency <0.01 in several databases: dbSNP137, 1000 Genomes (April 2012 data release), the National Heart , Lung and Blood Institute(NIH) Exome Sequencing Project (ESP) 6500 exomes, and 286 unrelated in-house controls.
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