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This method shows the feasibility of this analysis, with a less invasive approach.
Similarly, a cutoff of 200 μM was chosen to perform the analysis with a less stringent cutoff of active/inactive.
Given the trend, we reran the analysis with a less stringent correction for multiple comparisons (Sidak), yet the significance remained unchanged.
The number of families identified by CAFE as expanded along the internal cactophilic branch was reduced by considering only those families that were also found in expanded category after rerunning the analysis with a less stringent cutoff (35% amino acid identity, 50% coverage).
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Multivariable logistic regression (Table 3) confirmed the associations found in univariate analysis, but with a less marked relationship between response rate and country, as the lower response rate in studies in the US and Canada was partly explained by the larger size of these studies.
Variables with p less than 0.1 in univariate analysis were then used as independent variables in a stepwise logistic regression analysis, with a p less than 0.05 criterion for retention of variables in the final model.
We can many times complete an analysis with a bit less; we aim to load 1 ug total protein onto our system for each run but require the overage due to sample losses during preparation.
It is interesting to note that the only two samples, Ou 6 and Ou 8, judged EVOOs by the chemical analysis, with a slightly less FAEE content than the threshold value were classified as EVOOs or virgin olive oils by the organoleptic evaluation.
A set of primer pairs from G. barbadense EST sequences were run against the G. hirsutum EST sequences dataset based on in silico PCR analysis with a threshold less than 3 mismatch bases.
Then, the set of primer pairs from G. barbadense EST sequences were run against the G. hirsutum EST sequences dataset based on in silico PCR analysis with a threshold less than 3 mismatch bases.
The data analysis was repeated with a less strict case definition of 75% reduction in infectious centers relative to the positive control and including the 43 additional samples that could only be screened at higher dilutions.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com