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Total dendritic length, number of branches, average of dendrite length and Sholl analysis were quantified using NeuroStudio software (Icahn School of Medicine at Mount Sinai, NY).
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RNA expression was detected by Northern blot analysis and quantified using a Molecular Dynamics Storm 860 PhosphorImager/Fluorima. Protein expression detected by Western blot analysis was quantified using the ImageJ program.
Differential gene expression analysis was quantified using the Cufflinks package (v1.2.1) [ 73].
GLP1R expression analysis was quantified using the reference gene β-actin.
The level of methylation for each CpG within the target region of analysis was quantified using the Pyro Q-CpG Software.
After northern blot analysis, data were quantified using ImageJ software and total RNA levels were corrected for 5S RNA.
For quantitative analysis, respective bands were quantified using a ChemiImager™4400 (Alpha Innotech Corporation, San Leandro, CA).
For isoform analysis, aligned reads were quantified using the Cufflinks/CuffDiff package for paired-end settings with the Ensembl database and subsequently analyzed using CummeRbund in R [ 26].
Band intensities were quantified using Multi Gaugre analysis software.
Total protein levels of the samples were quantified using Bradford analysis prior to gel loading.
The immunoblots were quantified using densitometric analysis.
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