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Analyses of independent predictors for survival from univariate analysis were performed using a multivariate logistic regression.
Measurement of the electrochemical parameters and the subsequent analysis were performed using a µStat400 Biopotentiostat with a 4-electrode connector from DropSens, Spain.
Since the proposed method (Method 2) was adequate in all studied cases, an analysis of variance (ANOVA) and a response surface analysis were performed using a quadratic model for the mixed factors and a linear model for the process factors.
In order to identify predictors for death at 1 year of follow-up, a univariable analysis and a subsequent multivariable analysis were performed using a Cox proportional hazards regression model.
Fluorescence measurement and analysis were performed using a FACSCalibur and the CellQuest software (BD Biosciences).
Amplification and melt curve analysis were performed using a LightCycler (Roche Diagnostics, Indianapolis, IN).
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MS analysis was performed using a Waters xevog2qtof mass spectrometer.
The heterojunction analysis was performed using a parameter semiconductor analyser.
The elemental analysis was performed using a Vario ELIII.
Analysis was performed using a random-effects model.
Cell population analysis was performed using a Beckman Coulter cytofluorometer.
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