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After random mating for 300 generations and then selection on a complex quantitative trait over 200 generations with selection intensity 50%, followed by another 100 generations of random mating, the final traits for the analysis were generated using short-range to long-range epistatic marker effects.
The FCBs utilized within this analysis were generated using IGS final orbit and clock products.
Stocks used for overexpression analysis were generated using standard crosses.
It is important to note that the differentially expressed gene sets used for the top-down analysis were generated using all 53 microarrays.
Graphs and (where appropriate) statistical analysis were generated using GraphPad Prism software version 6.03.
All plots, graphics and data analysis were generated using in-house R scripts.
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A receiver operating characteristic (ROC) curve analysis was generated using the ELISA results for 491 sheep sera with the area under the curve (AUC) being 0.97.
Survival analysis was generated using the Kaplain-Meyer curve, and statistics were calculated using a log-rank test.
analysis was generated using DISTRUCT 1.1 [ 56].
The data analysis was generated using SAS/STAT software (1999).
The data analysis was generated using SAS software, version 9.3.
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