The phrase "analysis were determined using" is not correct in written English.
It should be "analysis was determined using" to match the singular subject "analysis" with the singular verb "was."
Example: "The analysis was determined using various statistical methods to ensure accuracy."
Alternatives: "analysis was conducted using" or "analysis was performed using."
The complete sequences of ORF2 of the three representative strains of the three major clusters in the phylogenetic analysis were determined using RT-PCR method with the primers designed according to the related sequences available in GenBank.
Denaturing acetonitrile gradient profiles and melting temperatures for analysis were determined using Wavemaker™ 4.1 software (Transgenomics).
The three different cut-off values in the analysis were determined using ROC analysis.
The significance of EMM screened in the stratified analysis were determined using Likelihood Ratio Test.
Extracellular amino acid concentrations for metabolic flux analysis were determined using a derivatization method and subsequent measurement in a HPLC system [ 54].
The appropriate model parameters for the maximum-likelihood analysis were determined using a likelihood-ratio test with Modeltest version 3.7 (Posada and Crandall, 1998; Posada and Buckley, 2004).
Statistical significance for parametric analysis was determined using unpaired two-tailed T-test or single-variable ANOVA, as specified in Supplementary table 2. For pairwise comparisons, a Holm Sidak test was used following ANOVA.
The amount of char used varied between 7 14% of total volume and its chemical analysis was determined using energy dispersive X-ray spectroscopy.
The morphological contribution of the film surface to the analysis was determined using atomic force microscopy for both cast and coagulated cellulose films.
Elemental analysis was determined using a Vario EL III Elemental Analyzer (Elementar, Germany).
Correlations analysis was determined using SAS 9.0 software, and charts were plotted using Sigma Plot 12 software.
