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Band patterns were scored in AFLP and ISSR fingerprint experiments and binary matrices for analysis were constructed for all populations of each species (POPGENE Version 1.32 [ 57]).

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A 4-component factor analysis was constructed for the Swedish and Danish samples of drug abusers.

A multiple regression analysis was constructed for each cognitive domain separately, with scores on the LSI-A as the dependent variable.

4. A 4-component factor analysis was constructed for the staff ratings on the subset of the Danish sample of substance abusers who were rated by staff members. 5. Canonical correlation analysis was used to assess the latent dimensions linking staff members' reports with DIP-Q scales.

SWISS-MODEL was used to analyze the molecular modeling of AsCOI1 protein.To determine the relationship between AsCOI1 and other COI1 proteins, phylogenetic analysis was constructed for 16 COI1 proteins of different species using MEGA version 5.05 by the neighbor-joining method with 1000 bootstrap replicates.

This was consistent with the fact that the small RNA and the degradome libraries for miRNA sequence analysis were constructed from developing maize ears (Additional file 14).

Total blood sample volumes for PK analysis were constructed by design not to exceed 1% estimated blood volume for safety, so that fewer samples for analysis were drawn from children < 2 years of age.

The calibration curves of SIL for HPLC analysis were constructed by plotting the peak area ratio of drug molecule to IS against the concentration of the drug.

Two logistic regression models for multivariate analysis were constructed with the binary dependent variable.

Multiple alignments for phylogenetic analysis were constructed using the MUSCLE program [ 98]; columns containing gaps in >30% of the sequences were discarded.

The cohort models used for the analysis were constructed by changing the screening protocol of the ERSPC-Rotterdam model to the screening protocols considered in this study.

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