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Colony images for Oct-4 quantities analysis were collected with fluorescent microscopy.

Data for this analysis were collected with an online survey targeting MSM living in Germany; the survey was online from 04/11/2013 until 13/01/2014.

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Immediately after slaughter, liver samples for gene expression analysis were collected, rinsed with sterile RNAse-free water solution, cut into small pieces (thickness of ~0.3 cm), stabilised in RNA Later solution (Qiagen, Hilden, Germany) and subsequently stored at –80°C.

OA and RA synovia for immunohistochemistry analysis were collected in accordance with experimental protocols approved by the Stanford University Institutional Review Board and with the patients' informed consent as described [ 12].

During year 2007 until March 2008, blood samples to be used for microarray analysis were collected at Carolinas Neuromuscular/ALS-MDA Center with approval by the IRB at Carolinas Medical Center.

CRC specimens for western blot analysis were collected and lysed with standard RIPA buffer (Boston Bio Products, Boston, USA), according to the manual, and were centrifuged at 10000 g at 4°C for 30 min.

The samples for the oxygen isotopic analysis were collected at 72 horizons with a 20-cm stratigraphic interval covering an 18-m succession (Fig. 2).

Tissues used in this study for mast cell analysis were collected after postmortem perfusion with 4% paraformaldehyde.

Clinical samples for analysis were collected from prostatectomy patients with full research consent at the Addenbrooke's Hospital, Cambridge, UK.

The MS and MS/MS data analysis were collected using a laser with an iteration rate of 1 kHz.

The data for this analysis were collected as part of Social Ties Associated with Risk of Transition into injection drug use (START), a longitudinal study, which aimed to identify social risk factors for initiating injection drug use among young adult non-injection and newly initiated injection drug users (heroin, crack, and cocaine) in New York City.

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