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P-values for replicative life span analysis were calculated using a 2-tailed Wilcoxon Rank-Sum test.
The diversity indices including AMOVA (analysis of molecular variance) based Fst analysis were calculated using ARLEQUIN 3.01 software [35].
All mean pixel density measurements from western analysis were calculated using Image J software.
Distant metastasis-free survival univariate and multivariate analysis were calculated using a Cox proportional regression model.
All qRT-PCR data for RNA expression analysis were calculated using the ΔΔCT method.
P-values for upstream analysis were calculated using the Fisher's exact test.
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Statistical analysis was calculated using Stata v. 11 using Student's t test for mean comparison.
The inter-observer agreement for the visual analysis was calculated using Cohen's κ.
Statistical analysis was calculated using Statistical Package for Social Sciences 12.0.1 for Windows (SPSS Inc., Chicago, IL, USA).
Statistically significant data were calculated using the signed rank test (p < 0.01), and agreement analysis was calculated using Cohen's kappa.
The interobserver agreement for the visual analysis was calculated using Cohen's κ for categorical variables and intraclass correlation coefficient (ICC) for quantitative variables.
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