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Prognostic variables identified by univariate analysis were analyzed using the χ-test.

The significant factors from the univariate analysis were analyzed using the Cox regression model.

Results of fragment analysis were analyzed using GeneMapper version 3.7 (Life Technologies).

Variables that were considered significant in then univariate analysis were analyzed using multivariate analysis.

Single nucleotide variations identified from paired tumor normal analysis were analyzed using the SIFT algorithm.

Comparisons of quantitative data from the proteomic analysis were analyzed using two-tailed Student's t-test.

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The statistical analysis was analyzed using one-way ANOVA.

Cost-benefit analysis is analyzed using a model of agency delegation.

Apoptosis analysis was analyzed using FITC-conjugated Annexin V and PI as described previously [40].

To analyze functional pathways that are coordinately regulated, the gene list generated by microarray analysis was analyzed using PathwayAssist software (Ariadne Genomics, Rockville, MD, USA), which is a software tool to generate biological association network.

The survival analysis was analyzed using the Kaplan Meier method, and significance was calculated using the log-rank test.

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