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Structure visualisation and analysis were achieved using PyMOL (DeLano 2002).

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Statistical analysis was achieved using nonparametric Mann-Whitney tests.

Elemental analysis was achieved using energy-dispersive X-ray spectroscopy on the FEI Tecnai G2 F20 U-TWIN.

Most information is obtained by cloning and sequencing the polymerase chain reaction (PCR) amplicons, but a more rapid analysis is achieved using fingerprinting techniques.

Independent statistical analysis was achieved using SAM (Significant Analysis of Microarrays, Standford University)[113].

Lead analysis was achieved using graphite furnace - atomic absorption spectroscopy (GF-AAS; AAnalyst 800, Perkin Elmer) using 50 µg of NH4H2PO4 and 3 µg of Mg(NO3 2 as matrix modifiers in each atomization.

Data analysis was achieved using the software progam GraphPad InStat.

Global gene expression analysis was achieved using Agilent 44 k human DNA microarrays containing 17596 unique gene symbols.

In the present study, QTL analysis was achieved using Maximum Likelihood with QTLMap software, in order to test the effect of the QTL analysis method on QTL detection.

Precursor selection for MS/MS analysis was achieved using the 4000 Series Explorer Software (AB SCIEX) with acquisition of the 20 most intense precursors (S/N > 20), beginning with the strongest first.

The best prediction of a future cardiovascular event, according to multiple Cox regression analysis, was achieved using a model that included previous coronary artery bypass surgery, prescriptions of statins and glucometabolic status.

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