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In addition, by using double mutant cycle analysis, we probed the energetic contribution of selected pairs of amino acid residues.
Using a range of methods such as atomic force microscopy, thioflavin T fluorescence, circular dichroism, and gas-phase electrophoretic mobility macromolecule analysis, we probed the propensity of human insulin amyloid to propagate or dissociate in a wide span of pH values and ageing in a low concentration regime.
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To confirm the identity of the bands observed in the gel-shift analysis, we performed a parallel analysis in which we probed the bands with an antibody specific to LFN.
Surface plasmon resonance analysis probed the interactions of 13 proteins with alginate-sulfate.
In the present study, we conducted computational molecular modeling study and biochemical analysis to probe the mechanism of COX activation by dietary bioflavonoids.
An uncertainty analysis was done, probing the sensitivity of the analysis to different assumptions.
In this analysis, we ranked the probes on significance and then selected 5% of the most significant probes that showed an at least three-fold change.
In a separate in silico analysis we matched the probe set to recently published enterobacterial genomes.
For pathway-level analysis, we mapped the probe sets to pathway lists by using hypergeometric enrichment analysis.
On the basis of the BADGE analysis, we selected the probe sets in which the probability of a fold change > 1.5 was either > 0.975 or < 0.025.
In this analysis, we excluded the probes targeting the sequences containing the common single-nucleotide polymorphisms (SNPs) or within 10 bp away from the common SNPs.
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