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The suitability of the analysis was verified using the Kaiser-Meyer-Olkin (KMO) measure.
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The formulae indicating the relationship between daytime vertical E × B drift velocity and ΔH, for the 'disturbed periods', obtained through the regression analysis were verified using the JULIA radar observed E × B drift velocity for 3 selected events.
The statistical assumptions for the regression analysis were verified using a residual analysis.
Significant findings from the IRR analysis were verified using VRP injury statistics software (University of North Carolina, Injury Prevention Research Center).
All conditions for survival analysis were verified using tests based on the Schoenfeld residuals and all assumptions were met.
Proportional hazards assumptions of the Cox regression analysis were verified using the 'survival' package in R using the cox.zph procedure (Grambsch and Therneau, 1994).
The motifs overrepresentation identified with Oligo-Analysis, was verified using POBO [ 31], which uses bootstrap to verify the statistical overrepresentation of a given motif.
For expression analysis, normality was verified using the Shapiro-Wilk normality test.
RNA purity and concentration were assessed using spectrophotometric analysis, and integrity was verified using gel electrophoresis.
Similar transfection efficiency (50%) was assessed by FACS analysis and cell viability was verified using trypan blue.
Sox2 expression was validated by immunoblot analysis and Cre expression was verified using a human embryonic kidney 293T (HEK293T) reporter system that was used to calculate viral titer.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com