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Microarray analysis was validated in qPCR studies using human postmortem brain tissue and CA1 sector and regional hippocampal dissections obtained from a mouse model of AD/Down syndrome (Ts65Dn mice) and normal disomic (2N) littermates.
Each statistical analysis was validated in 1000 bootstrap samples.
Quantitative Ho SPECT, necessary for dosimetric analysis, was validated in a realistic model.
Therefore our functional analysis was validated in four independent experiments, by testing two genes in two conditions.
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Only two of the SNPs that were found to be significantly associated with T2DM in the case-control analysis were validated in the prospective study.
In addition, survival and time-to-readmission estimates generated in the cohort analysis were validated in the matched-pairs analysis.
Independent predictors of survival identified on multivariate analysis were validated in an independent, stage-matched cohort of 68 patients.
The effects of significant markers obtained in the interaction analysis (see below, Association mapping and interaction analysis) were validated in other germplasm arrays of Barley CAP breeding lines.
Results from unsupervised analysis were validated in six external adenocarcinoma data sets (n=687), and six data sets comprising normal airway epithelial or normal lung tissue specimens (n=467).
More importantly, the results of univariate survival analysis were validated in an independent set of patients, using the same cutoff points of MCM expression as in the population study.
The HPLC analysis was validated for both test substances in selectivity, linearity, accuracy, precision and stability.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com