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The COD of filtrated (0.45-μm polyethylene terephthalate (PET)) raw waste water samples, which were stored frozen before analysis, was quantified with cuvette tests (Hach-Lange, Düsseldorf, Germany).
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Immunogold labelling was quantified with analySIS (Soft Imaging Systems) from digital images of two sections from two mouse neocortices (50 mitochondria from each section) acquired in a blinded manner.
The amount of proteins was quantified with densitometry analysis using Image J. Detailed information of antibodies used for Western Blot analysis are available in Supplementary Information.
The hydration products and the microstructure of the resulting pastes were analysed by means of FTIR and BSE SEM and the degree of hydration was quantified with Image analysis.
The first part of the infestation dynamics assessment was quantified with correlation analysis between different degrees of locust infestation risk in individual years and climatic variables.
The intensity of the bands was quantified with the image analysis software ImageJ 1.44p (National Institutes of Health, Bethesda, MD, USA), and the densitometry analysis is shown in arbitrary units normalized to the GAPDH loading control.
Temporal scaling was quantified with detrended fluctuation analysis.
Activation of MAPKs was quantified with Image J digital analysis software.
The TLR4 protein level was quantified with an image analysis program from the microscope system.
The mean green intensity of the nuclear staining was quantified with Lucia® image analysis software in 10 randomly selected cells.
The infiltration of MPO-positive cells was quantified with TissueQuest cell analysis software (TissueGnostics GmbH, Vienna, Austria) by counting the positively stained cells in nine inflamed areas with leukocyte infiltration from three different mice with each genotype.
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