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The specific analysis was programmed with the operating software MARS.
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SigmaScan is a flexible package for image analysis and SigmaScan Pro can be programmed with a specific macro language.
For this analysis, the pyrolyser was programmed at 650°C with He as the carrier gas.
Image fluorescence quantification analysis was performed with program MetaMorph (Version 7.7.3.0) and TUNEL assays.
All the analysis was performed with program SPSS 12.0 (SPSS Inc., Chicago, IL, USA).
Image processing and analysis was performed with programs written in-house in C and IDL Research Systems Incc, Boulder, CO, USA), running on a unix workstation.
The power analysis was performed with PS Program (Power and Sample Size Program: http://biostat.mc.vanderbilt.edu/wiki/Main/PowerSampleSize) [ 34].
Multivariate statistical analysis was performed with R program environment by using an in-house program.
The analysis was performed with ImageJ program.
Statistical analysis was conducted with R programming language.
General data analysis was conducted with R programming language.
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