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Univariate analysis was preformed, separately, on each morphometric parameter, using ANOVA (analysis of variance), with STATISTICA software.
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Statistical analysis was preformed with SAS 9.2 (Cary, NC) for Windows.
To confirm that ASL3 was the gene associated with the phenotype observed, RNA interference (RNAi) analysis was preformed.
Statistical analysis was preformed using SPSS 13.0 for MicroSoft™ Windows.
All slides were kept at 4°C in dark until confocal microscopy analysis was preformed.
Statistical analysis was preformed using student's t-test (SPSS. Chicago, IL).
Loci-spanning probe melt analysis was preformed by continuous signal acquisition between 38°C – 65°C at default ramp rate of 0.6°C/s and 5 acquisitions per °C.
This analysis was preformed by dividing the experiment into blocks (each comprising the same number of stimulus representations) and comparing the response amplitudes across blocks.
TLC analysis was preformed as in ref (49).
The analysis was preformed using Sigmastat 3.5 (Systat Software, San Jose, CA, USA).
Sulfation analysis was preformed as described previously [ 22].
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