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The statistical analysis was performed utilizing an analysis of variance (ANOVA).

Quantitative confocal analysis was performed utilizing the Zeiss LSM software to scan individual CTEC and mTEC nuclei to determine the mean fluorescence intensity of Foxn1 antibody staining in ST control and TetO-Dkk1 sections.

To assess the levels and distribution of HIV-1 subtype C RNA in plasma, the analysis was performed utilizing existing data from three types of cohorts: general population, MTCT, and cART-initiating cohorts.

Gene expression analysis was performed utilizing Affymetrix DNA-microarrays, realtime PCR and Western Blot.

Statistical analysis was performed utilizing the freely available software program R version 3.2.0 [ 48].

Statistical analysis was performed utilizing JMP 11 (SAS Institute Inc., Cary, NC, USA).

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Cluster analysis were performed utilizing the Neighbor-Joining method [24] on the basis of a distance matrix calculated with the Bionumerics software (version 3.5).

In order to explore which ecological classes are most responsible for separating assemblages, a principal components analysis (PCA) was performed utilizing a covariance matrix.

Fixed factor analysis of variance was performed utilizing the continuous scale data to inspect variation of dental anxiety across major demographic, behavioural and socioeconomic status factors.

In the ELK1 knock-down experiments, the relative quantitative analysis in binding activity was performed, utilizing densitometry and statistical analysis, by comparison of siELK1 with non-specific siRNA control, and the control was set at 1. ZW and PAB designed the experiment.

A western blot analysis of KCNMA1 expression was performed, utilizing the same cell lines previously examined by northern blot (Figure 2B) for KCNMA1 transcript expression.

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