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Proximal tubule anchor genes network and GO analysis was generated by Ingenuity Pathway Analysis (http://www.ingenuity.com), using default settings and gene interactions curated in the Knowledge Base database.

After that, a matrix for multivariate data analysis was generated by the software with a mass clustering window of 0.005 Da and the retention time clustering window of 0.2 min. The matrix covered the information of the retention time, m/z and the ion intensity for each picked peak.

Pathway analysis was generated by Ingenuity IPA software.

Statistical analysis was generated by SAS 9.1.2 (SAS Institute, Cary, North Carolina).

A neighbor-net network analysis was generated by using SplitsTree 4 software (19 ).

The read count per gene for analysis was generated by featureCounts from the subread package [ 43].

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CCA analysis were generated by Canoco 4.5 to evaluate the specific correlations between characteristic genera and environmental factors measured in the study, including iron, manganese, ammonia, DO, temperature, pH value and layer height.

The data required for regression analysis is generated by solving the transient heat and mass transfer equations of adsorbed natural gas (ANG) storage system undergoing charging under constant pressure condition.

The data required for regression analysis is generated by numerically solving the transient heat and mass transfer equations of adsorbed natural gas (ANG) storage system undergoing discharge under constant flow condition.

For Archaea, 16S rRNA gene fragments ∼190 bp long for DGGE analysis were generated by nested PCR.

Cell pellets were lysed using PBS supplemented with 1% Igepal, protease inhibitor, 2 mM DTT and soluble lysates for immunoblot analysis were generated by centrifugation at 13,000 xg for 15 min. When indicated, culture supernatants are run on SDS PAGE for analysis.

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