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The expression of 129 genes (Table S1) including 31 DNA repair genes and 38 high or low variability genes from the Illumina HT-12 expression data analysis was determined using NanoString nCounter Gene Expression platform (NanoString Technologies, Seattle WA) at the University of Miami Oncogenomics Core facility as previously described [ 30, 31].
Statistical analysis was determined using Students t-test using Excel (Microsoft, Redmond, WA, USA) or ANOVA followed by Post-hoc t-test and Bonferroni correction.
Elemental analysis was determined using a Vario EL III Elemental Analyzer (Elementar, Germany).
Correlations analysis was determined using SAS 9.0 software, and charts were plotted using Sigma Plot 12 software.
The morphological contribution of the film surface to the analysis was determined using atomic force microscopy for both cast and coagulated cellulose films.
The amount of char used varied between 7 14% of total volume and its chemical analysis was determined using energy dispersive X-ray spectroscopy.
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The complete sequences of ORF2 of the three representative strains of the three major clusters in the phylogenetic analysis were determined using RT-PCR method with the primers designed according to the related sequences available in GenBank.
The three different cut-off values in the analysis were determined using ROC analysis.
Denaturing acetonitrile gradient profiles and melting temperatures for analysis were determined using Wavemaker™ 4.1 software (Transgenomics).
The significance of EMM screened in the stratified analysis were determined using Likelihood Ratio Test.
The appropriate model parameters for the maximum-likelihood analysis were determined using a likelihood-ratio test with Modeltest version 3.7 (Posada and Crandall, 1998; Posada and Buckley, 2004).
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