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We aimed to determine the predicted infliximab trough concentrations in children with CD during maintenance therapy and the percentage of patients achieving target trough concentration >3 μg/ml.A Monte Carlo simulation analysis was constructed using a published population pharmacokinetic model based on data from 112 children in the REACH trial.

The spatial scan analysis was constructed using postal code data for participants (n = 154) residing in the Vancouver Metropolitan Area.

Through the discussed analyses, the flow of the sequential analysis was constructed using FT-IR, ToF-SIMS, NanoSIMS, Raman spectroscopy, XANES and TEM/STEM to minimize sample contamination during analysis (Table 3).

A neighbour-joining tree with K2P distances from all haplotypes from species found in pyrosequencing analysis was constructed using Mega 4.1 [25].

The genetic relationships between different sequence types were determined using eBURST [46] and a phylogenetic tree based on neighbor-joining analysis was constructed using the Phylip package (http://evolution.genetics.washington.edu/phylip.html).edu/phylip.html

Phylogenetic analysis was constructed using the Mega 4 software [ 16].

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Box plot graphics and statistical analysis were constructed using software SigmaPlot Version 11.0 (Systat Software, Inc., Chicago, USA).

Multiple alignments for phylogenetic analysis were constructed using the MUSCLE program [ 98]; columns containing gaps in >30% of the sequences were discarded.

A decision analysis model was constructed using Excel.

Prior to quantitative analysis, a standard curve was constructed using serial dilutions of RT product and the efficiency of each primer set was determined using the equation [(10(−10slope)-10·100].

The 34 quantitative and qualitative morphometric traits were used for cluster analysis and a dendrogram was constructed using the nearest-neighbour and complete-linkage methods (see Fig. 4).

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