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Since normal tissue (required for LOH analysis) was available from 86 patients only, a gene copy number analysis was performed by quantitative PCR using hydrolysis probe-assays (LightCycler 480 system; Roche).
DNA of adequate quality and quantity for analysis was available from 35 of the 52 patients treated on study, and both SNPlex and direct sequencing data were generated for these individuals (see Table 1 for demographics and pre-treatment characteristics of this patient subset).
Simple usage analysis was available from Google Analytics data.
KEGG pathway analysis was available from http://www.genome.jp/kegg/pathway.html.html
For technical reasons, high-quality DNA for analysis was available from 294 patients only.
Left-over DNA for SYBR Green real-time PCR analysis was available from 12 normal lung tissues and 11 lung cancer tissues (4 adenocarcinoma, 4 squamous, and 3 small cell lung carcinoma).
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Solid on-the ground analysis is available from the International Crisis Group, a non-profit group dedicated to the resolution of conflicts.
The dataset and the R code used in the empirical analysis is available from the author upon request.
Sufficient amounts and quality of pre- and post-treatment plasma samples for miR analysis were available from 8 of 12 patients.
The list of primers used for mutational analysis is available from the authors upon request.
Data for this analysis were available from 12, 25 and 2 experiments in cotton, maize and potato, respectively.
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