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Spectral images for sRET analysis was acquired as described earlier [16].
Analysis was acquired on an LSR II (BD Biosciences) and analyzed using the FlowJo software.
The first post-bleach frame used for the downstream analysis was acquired 2 min after photobleaching to allow for complete equilibration of bleached soluble SMC1-EGFP across the nucleus.
Data analysis was acquired by AB Sciex Analyst 1.5 workstation and based on spectral MS/MS library match, with additional information from reference journal, and utilizing advanced chemometrics software data processing and interpretation with fragmentation prediction (ACD/Labs MS Fragmenter).
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EELS spectra for the elemental analysis were acquired from the CNF shown within the broken black circle in Figure 2a.
Sizing of unit operations, chemicals and utility consumptions, and estimation of capital and operating costs with the cost breakdown analysis were acquired from the simulation.
The necessary parameters for time domain interpolation re-sampling, which is totally based on the kinematic analysis, are acquired from the fitting curve and the re-sampling sequence could be established in the time domain.
Images used for analysis were acquired with a Zeiss (Oberkochen, Germany) LSM510 confocal microscope in 1 µm optical sections.
In brief, 1D 1H NMR spectra for SCS analysis were acquired using a Bruker Biospin Avance 400 spectrometer.
The data presented in this analysis were acquired retrospectively and anonymously.
Samples for water parameter analysis were acquired from the same reactor and at the same time as the microbial samples.
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