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Data analysis was achieved using the manufacturer's online services.
Statistical analysis was achieved using nonparametric Mann-Whitney tests.
Elemental analysis was achieved using energy-dispersive X-ray spectroscopy on the FEI Tecnai G2 F20 U-TWIN.
Independent statistical analysis was achieved using SAM (Significant Analysis of Microarrays, Standford University)[113].
Lead analysis was achieved using graphite furnace - atomic absorption spectroscopy (GF-AAS; AAnalyst 800, Perkin Elmer) using 50 µg of NH4H2PO4 and 3 µg of Mg(NO3 2 as matrix modifiers in each atomization.
Data analysis was achieved using the software progam GraphPad InStat.
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Most information is obtained by cloning and sequencing the polymerase chain reaction (PCR) amplicons, but a more rapid analysis is achieved using fingerprinting techniques.
Structure visualisation and analysis were achieved using PyMOL (DeLano 2002).
The biological interpretation and simultaneous statistical analysis of these complex data necessitated the development of new representation and analysis tools, which was achieved using custom Perl scripts [ 18, 20].
Quantitative analysis of sphingolipids was achieved using selective reaction monitoring scan mode.
16, 20 Analysis of sequence traces was achieved using Variant Reporter v1.1 (Life Technologies) and by manual inspection of electropherograms.
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