The theoretical T-RF values of the representative phylotypes represented in the clone library were calculated using the NEB cutter [36] and confirmed experimentally by T-RFLP analysis using the corresponding clones as templates.
To generate a complete list of all the members of the tomato RLK family, we performed a phylogenetic analysis using the Arabidopsis family as a template.
The nucleotide changes were determined by polymerase chain reaction-restriction fragments length polymorphism (PCR-RFLP) analysis using the isolated genomic DNA as a template.
Digitizing the raw neural signal allowed for post-hoc analysis using the Spike2 software that provides software window discriminators and level detectors as well as various forms of waveform analysis including template matching and spike sorting, using PCA algorithms.
Interviews were analysed using the template analysis style of thematic analysis [ 28], a systematic technique for categorising qualitative data in hierarchical clusters.
Using the template analysis approach [ 18], a flexible coding structure was initially developed based on the major components of the Expanded CCM (self-management/develop personal skills, delivery system design/re-orient health services, decision support, information systems, build healthy public policy, create supportive environments; and strengthen community action) [ 9].
These data confirm that mRNA contamination from other cells was not a problem.Next, we estimated the sensitivity of the single-cell nested RT-PCR analysis using template cDNA, the copy number of which was known.
Analysis involved a) reading the full transcripts to obtain a sense of the overall data; b) conducting a thematic analysis using a template of codes based on the key components of an interprofessional approach to SDM and open codes for the new themes that had been inductively derived; and c) comparing coders' findings to reach agreement about the main themes identified.
Field notes and other documents (practice applications; workshop reports; final plans describing practice-specific outcomes) provided a substantial basis for thematic analysis using a template approach developed during data collection [ 23].
This genomics tool offers the opportunity for large-scale biological approaches combining both expression and genomic-level sequence analysis using as a template an entire vertebrate genome.
