First, we compared the expression of NMDAR1 receptor by western blot analysis using cells lines from different origins including SH-SY5Y (neuroblastoma), HCT-116 (colon colorectal carcinoma), and three hepatocarcinomas (HepG2, HuH-7, and HLF).
Most studies on heterocyst patterning were based on statistical analysis using cells collected at different times from a liquid culture.
Most previous studies on heterocyst patterning were based on statistical analysis using cells collected or observed at different times from a liquid culture, which would mask stochastic fluctuations affecting the process of pattern formation dynamics in a single bacterial filament.
In a first approach the F-E2G5 antibody was examined for its substrate specificity by western blot analysis using cell extracts from HEK293 cells expressing GFP, YB-1-GFP or YB-1(21-147 -GFP proteins.
Immunoblot analysis using cell lysates from CPBF1gs-EhCP-A5-HA, in which CPBF1 expression was repressed by gene silencing, showed that EhCP-A5-HA processing was abolished (Fig. 6A, right panel).
All the analysis were made using cells from at least four different fish.
It might be important that the accessibility analysis used cell lines derived from transgenic mice, where the DNA passed through the germ line and was subject to dense methylation.
ELISpot analysis was performed as previously described 45, using cells from pericardial fluid and blood samples.
ELIspot analysis using spleen cells from immunized offspring of vaccinated mothers was performed (Table 1).
