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Plasma was stored at −20 °C until analysis using a validated LC-MS/MS analytical method.
Blood and peritoneal samples were immediately centrifuged and separated in total and ultrafiltered (Uf) fractions, then frozen until analysis using a validated method based on flameless atomic absorption spectrophotometry using a Varian SpectrAA 220Z graphite furnace spectrometer with Zeeman effect (Varian, Mulgrave, Australia).
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We performed a case-by-case analysis, using a previously validated instrument which covered five broad domains: healthcare organization, communication between healthcare providers, patient risk factors, clinical management, and clinical outcomes.
Fully automated analysis using a Celigo cytometer was validated for tumor spheroid growth and invasion against standard image analysis techniques, with excellent reproducibility and significantly increased throughput.
The general approach was to conduct a baseline CFD analysis (using a range of simplifications/assumptions) and validate this using a physical experiment before using CFD alone for iterative design improvement.
We then performed a sensitivity analysis, using only independently validated cancers, which did not substantially alter the site-specific results (data not shown).
Design Retrospective analysis using IMPACTSEC, a previously validated policy model, to apportion the recent decline in coronary heart disease mortality to changes in major cardiovascular risk factors and to increases in more than 40 treatments in nine non-overlapping groups of patients.
Engineering analysis using FEA technique validate the high strength of the structure.
The analysis used previously validated scripts written in the R language [ 21].
Pharmacokinetic analysis was performed using a fully validated HPLC UV method (Stopeck et al, 2002).
Pyronaridine sample analysis was performed using a previously validated LC-MS assay; details regarding the assay, including precision, accuracy, stability, and recovery data, were previously published (3).
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