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In the current study, according to a quantitative real-time PCR analysis, the expression level of miR-224 was lower in CRC specimens compared to normal tissues.
Semi-quantitative RT-PCR was performed for analysis the expression level of CaPAO gene in transgenic tobacco, and the RD29A-F and CaPAO-R primer pair were used.
In an unsupervised analysis, the expression level of TCF signature was significantly increased in CMS-induced cells (t-test, P=0.0014, Figure 4a).
Consistent with the RLCS analysis, the expression level of SLIT1 mRNA was generally high in the sensory and prefrontal association areas and low in the primary sensory areas.
By using quantitative real-time PCR (qPCR) analysis, the expression level of OsCNGC genes was investigated in leaves of the rice cultivar Nipponbare (O. sativa L. ssp. japonica).
Based on the northern blot analysis, the expression level of Gma-m001 decreased under salinity stress, but identical patterns were observed under drought and alkalinity when compared with Solexa sequencing.
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In addition to the profiling analysis, the expression levels of ten DEGs were validated by quantitative reverse transcriptase-PCR.
By use of QRT-PCR analysis, the expression levels of the virR genes in these strains were shown to be significantly higher than in the wild-type strain (Fig. 4B).
In univariate analysis, the expression levels of TGF-β1 (P = 0.016), fascin (P = 0.006), NF-κB p105 (P = 0.022) and PKC-ζ, (P = 0.042) were significant indicators for disease specific survival (DSS).
In the expanded analysis, the expression levels of these 10 genes were still significantly different with absolute fold change >4, p≤0.05 and consistency >85%, except genes SPP1 and NDRG2, which exhibited slightly decreased consistency (Figure 1).
For analysis, the expression levels of all target genes were normalized to GAPDH expression (ΔCt).
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