In addition to the profiling analysis, the expression levels of ten DEGs were validated by quantitative reverse transcriptase-PCR.
To analysis the expression of CsPI in wild-type and pi-1 mutant A. thaliana, the A. thaliana housekeeping gene ACTIN was used as normalization control.
In our analysis, the expression of Ghd7, reported to regulate photoperiodic flowering (Xue et al. 2008), did not show significant alterations in its transcript levels over different developmental stages (Figure2C).
The third kind was several promoters of genes involved in stress response, because on account of transcriptome analysis, the expression of several stress response genes, i.e. dnaK, clgR, clpB, trxB1, dnaJ and sufR, up-regulated significantly during GABA conversion stage.
Open image in new window Open image in new window Figure 2 Statistical analysis the expression difference of miRNAs in paired organs of amphioxus, and validation of the expression of tissue-specific miRNAs.
Furthermore, to test whether the mechanisms were associated with up-regulating of GLT-1, we used a GLT-1 specific inhibitor dihydrokainate (DHK) and analysis the expression of GLT-1 mRNA and protein in cortex of brain.
We have then assessed by CLSM analysis the expression of the transcriptional factors T-bet and RoR-γt, specific for the Th1 and Th17 subsets, respectively.
In this analysis the expression patterns of distinct first exons served as a proxy for the activity of the promoter and all associated distal regulatory elements.
Among the 13 different NK cell markers studied by flow cytometry analysis, the expression of CD244 and NKG2D increased significantly in the CD56bright NK population.
ROC curve analysis The expression profile of each identified miRNA was used as the input for Receive Characteristics Curve (ROC) analysis with the ROCR package [32].
In our microarray analysis, the expression of 11 rat homologous NR members was permanently altered by perinatal protein undernutrition (Table 4).
