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In order to better characterize the multinucleated cells formed in response to IL-32, we investigated, using Western blot analysis, the activation of downstream signalling pathways.
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Promoter analysis revealed the activation of GLI-1 promoter activity in RANKL-overexpressing cells, suggesting that activation of GLI-1 is due to RANKL.
Analysis of the activation of PLK1 [ 25], using an antibody which recognizes the active form, showed similar staining in RASSF7-knockdown and control cells.
Immunoblot analysis confirmed the activation of caspase-3.
IGMA analysis indicates the activation of the 〈1 1 1〉{1¯10} slip mode.
Electrochemical analysis of the activation of dioxygen in aprotic solutions (acetonitrile) by manganese porphyrin polymer films was investigated by rotating disk electrode voltammetry.
Immunoblot analysis confirmed the activation of the NF-ĸB signaling pathway as SF induced protein expression levels of IKKα, IKKβ, p65, and the degradation of IκBα.
Deep transcriptome analysis showed the activation of a set of pluripotency and germ-cell-specific markers and the downregulation of innate immune system.
Also included is the quantitative analysis of the activation of the nuclear transcription factor κB (NF-κB) (A) and the tissue expression of heme oxygenase-1 (HO-1) (B) and cleaved caspase-3 (C) from immediate postmortem kidney specimen in the vehicle-treated (open box plots, n = 11) and HAM1101-treated (grey box plots, n = 9) mice.
Isolated ECs from WT and CD146EC-KO mice were starved with DMEM medium for 24 h and then induced with VEGF (50 ng/mL) at 37°C for 10 min, 30 min or 7 h for analysis of the activation of VEGFR-2, p38/AKT/ERK and NF-KB, respectively.
Further analysis revealed the activation of both apoptotic pathways via modulation of the proteins involved in the extrinsic and intrinsic pathways with an increase in TNF-R1, Fas-L and Bax levels and a reduction in Bcl-2 expression.
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