We also conducted GOMiner analysis of the genes that were removed from the original 1193-transcript toxic Se dataset due to overlap with genes altered by general toxicity and/or calorie restriction.
Semi-quantitative RT-PCR analysis was performed on AC explants that were removed from control (non injected, RT) and injected embryos cultured until the neurula stage 16.
For each time course, three red vertical lines represent the events (a), (d) and (e) described in Figure 2. The ICs that were removed from analysis are labeled (see Table 1 ).
For this analysis, solvent was removed to produce Ag/A.
For STAT3 phosphorylation analysis, food was removed 18 h before ICV injection.
For the analysis, patient identification was removed.
EDTA tubes were centrifuged and a cellular layer with the intention of later DNA analysis was removed.
DNA content analysis showed that nuclear material was removed from the BSM scaffold.
The main difference from the first analysis is that, once F2 was removed, the differences between 15 p, 30 p and 60 p all became more distinctive.
Samples that were obvious outliers according to Hotelling's T2 values [ 19] in the cluster analysis and PCA analysis were removed from further analysis.
