For flow cytometric analysis, spheres were filtered through a 40-μm cell strainer (Becton Dickinson, Heidelberg, Germany) and treated with Accutase (Sigma-Aldrich) to obtain a single cell suspension.
Please note that the following objects were not included in our analysis: (a) sphere S0 was excluded due to its very small size, (b) bottle C1 was not analyzed because we discovered that a large error was made in determination of its activity, and (c) sphere S1 was not analyzed because it was not visible in images reconstructed from scans with hot water.
For analysis of sphere formation, subconfluent cells were thoroughly dissociated with EDTA to prepare a single-cell suspension that was always visually verified.
Thus readers will encounter many references to, and, frequently, extended disquisitions on, such matters as Hamilton's Quaternions, Gibbsian vector analysis,Riemann spheres, Prandtl's discovery of the boundary layer, the Hilbert Pólya Conjecture, the Minkowskian space-time track, and Zermelo's Axiom of Choice.
(C ) qRT-PCR analysis of spheres infected with Ad-eGFP (black) or Ad-4TF (gray) followed by extended culture.
Among the most popular are limited dilution analysis, and sphere-formation assay., Biochemical markers of TISCs, which can vary widely between tumor types and individual tumors are routinely used in experimental oncology applications.
Then, we conducted cDNA microarray analysis on spheres from ovarian A2780 cells, and from breast MCF7 and SUM159 cells, and found that molecular pathways underlying spheres from these cancer cell lines were similar to a large extent, suggesting that similar mechanisms are involved in the genesis of CSCs in both ovarian and breast cancer types.
A classification is given of some differential capacity curves based upon a mean field analysis of a hard sphere ion dipole mixture adjacent to a uniformly charged plane hard wall with specific dipole adsorption.
This article proposes the spherical deconvolution of the fiber orientation density in a reproducing kernel Hilbert space, thereby generalizing previous approaches that perform a truncated Fourier analysis on the sphere.
The time window of our analysis spans from sphere to 50% epiboly stages, a period during which different forces may contribute to epiboly.
