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For spine analysis, slice cultures were infected with Sindbis virus expressing EGFP on DIV 12 in culture and fixed on DIV 15 with 4% paraformaldehyde/sucrose.
Five different maneuvers (low-flow inflation [ 28], incremental positive end-expiratory pressure trial (PEEP wave [ 29]), enlarged tidal volume breath for dynamic pressure-volume analysis (SLICE method [ 30]), static compliance by automated single steps [ 31] and super-syringe [ 32]) were performed in random sequence.
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He noted that the manuals state that they can be changed by the university at any time unilaterally and said Mr. Giuliani's overall legal analysis "slices far from the fairway".
Based on this analysis, slices are being extracted from the source image.
For histochemical analysis slices were processed with hematoxylin-eosin staining.
For CD68 immunohistochemical analysis, slices were incubated in methanol/H2O2 (0.3% vol./vol)./vol
For morphological analysis, slices of about 2 mm thickness were cut in isotonic saline perpendicular to the brain surface, followed by immersion-fixation in 4% paraformaldehyde (PFA) in 0.1 M phosphate buffer (PB), pH 7.4, for 24 - 48 h at 4°C and by cryoprotection in 20% sucrose in PB overnight.
For histochemical analysis, slices were stained with hematoxylin-eosin and Bielschowsky reaction, a silver staining for axons (nerve fibers are sensitized with a silver solution, the sections are treated with ammoniacal silver, and then reduced to a visible metallic silver) [ 54].
ANDREW GELMAN has done fascinating work using statistical analysis to slice and dice the electorate and figure out what makes it tick, and today he turns that analytical eye toward Tuesday's presidential election.
Initial analysis included slice time correction and Motion realignment.
AS carried out most of the electrophysiological analysis of slice cultures.
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