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For chemical analysis, samples were processed according to the methods described in APHA.
GC/MS analysis samples were processed essentially as described in [ 77, 79].
For miR analysis, samples were processed and hybridized to Affymetrix miRNA 3.0 arrays (Affymetrix, Santa Clara, CA).
For standard immunohistochemical and immunofluorescent analysis, samples were processed and embedded in paraffin and sectioned at 4 μm.
For mRNA analysis, samples were processed and hybridized to Agilent ZebraFinch Oligoarray v2.2 (Agilent, Santa Clara, CA).
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The cDNA was used for real-time RT-PCR analysis or samples were processed for library preparation.
For immunohistochemical studies, preliminary experiments were conducted to determine the effect of time delay on the outcome of immunohistochemical analysis, ensuring that samples were processed within an acceptable time frame.
All blood donors provided written informed consent for the collection of samples and subsequent analysis and the blood samples were processed anonymously.
For single sample LC-MS/MS analysis (n=3 per group), samples were processed essentially as described (Tonack et al, 2010).
In relation to serum fatty acid composition, one sample was discarded due to problems in its analysis; therefore only 21 samples were processed.
The ICTR distributed samples for immediate analysis to the appropriate laboratories; samples were processed and aliquots of whole blood, plasma, and serum were stored at −70 °C for future analysis.
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