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For desensitization analysis, plants were first treated with 10 mM Glu for aequorin luminescence imaging.
For biomass and RWC analysis, plants were separated into shoots and roots.
For the QTL analysis, plants were grown in a greenhouse (average air temperature, 30°C; average relative humidity, 50%; natural lighting).
For phytohormone analysis, plants were grown as described above.
For further analysis, plants with intermediate activity in mature leaves were chosen.
For Affymetrix analysis, plants of each cultivar were infested with 10 aphids and incubated for 48 h.
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For endogenous BR analysis plant materials (4 g fresh weight) were lyophilized and grinded.
In the final analysis, plant species was the unit of replication.
CK carried out protein extraction, and 2 D gel analysis, plant inclination experiments and apical curvature measurements.
According to the model-based clustering analysis, planted individuals are classified into two main clusters, green and pink.
Prior to biochemical and hormonal analysis, plant samples were freeze-dried using a Virtis Freeze Dryer (Gardiner, NY, USA) for 4 7 days while for other experiments (such as mRNA expression analysis) fresh plant samples were used.
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