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(F) Quantitative analysis of differentially expressed protein spots from (E). (G) Analysis of translation efficiency in vivo by 35S-methionine incorporation.
In the proposed method, combined with a one-dimensional codebook from block coding, a decoding method based on the minimum hamming distance is designed for analysis of translation efficiency.
The availability of SITS will facilitate the analysis of translation mechanisms in different cell-free system and direct comparison of cell-free systems with each other.
Autoradiographic analysis of translation reactions resolved by SDS PAGE, using the reticulocyte lysate alone, indicated that in vitro-transcribed mRNA drove the expression of a 46 kDa radiolabeled protein (Fig. 2A).
Analysis of translation, the next step in the processing of genetic information, reveals more prominent and informative differences between cells, and correlates more closely with protein levels [32], [34].
Mutation analysis of translation initiation factors shows that translation initiation factors of the eIF4E family are determinants of plant susceptibility to RNA viruses and viruses have adopted strategies to use different isoforms.
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This study described a global analysis of translation-level regulation in a model bacterium (L. lactis).
Likewise, a very deep-branching position for the genera Micrarchaeum and Parvarchaeum based on their gene content with both typical euryarchaeotal and crenarchaeotal genes (Baker et al. 2010) has not been validated by phylogenetic analysis of translation-related proteins, which placed them nested within the Euryarchaeota (Brochier-Armanet et al. 2011).
Lastly, the recent application of the ribosome profiling technique [101] to analysis of organellar translation [102] promises to revolutionize investigations of regulation of mitochondrial translation.
Analysis of the translation products showed three major bands which corresponded to the three predicted translation products and their molecular weights: uncleaved [GFP-F2A-CherryFP] polyprotein and the two major cleavage products, [GFP-F2A] and CherryFP.
We applied ribosome profiling and mRNA-seq techniques at the gene-level and codon-level analysis of the translation efficiency in E. coli and showed that genes with high codon adaptation shows higher translation initiation efficiency, as well as higher translation elongation efficiencies.
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