Sentence examples for analysis of transformants in from inspiring English sources

Lastly, we used transformed, hygromycin B-selected mycelia for the identification of the genomic sites of integration by TAIL-PCR (Liu and Whittier [1995]), a widely used approach for the analysis of transformants in systems where homokaryotic colonies are hard to obtain (Poma et al. [2006]).

For the analysis of transformants, cells were grown in Erlenmeyer flasks of 100 mL capacity at 25°C under continuous illumination (50 μmol photons m−2 s−1) in liquid TAP medium.

Procedures for S. gordonii transformation, screening and genetic analysis of transformants were as previously described [ 33].

Southern blot analysis of transformants indicated that the plasmid vector was randomly integrated in multiple copies into the genome of C. acutatum.

c) Integration site sequences retrieved from TAIL-PCR analysis of transformants #2, #8, #10, #11 and #12; The underlined and highlighted in bold sequences represent the portion of the T-DNA adjacent to the unknown sequence obtained by PCR.

An immediate phenotypic analysis of transformants is made possible by the predominance of the haploid gametophytic state in the life cycle of the moss.

The karyotype analysis of transformants showed that chromosomes XIV, III, and IV, or other chromosomes are split.

Southern analysis of transformants for bgl2 analysis.

Molecular analysis of transformants Genomic DNA of the transformants was extracted using a phenol chloroform method (Seiboth et al. 2002).

Agarose gel analysis of transformants showed that they carried only a single, large plasmid.

PCR analysis of transformants indicated that a single copy of RFP-hygMX had integrated at MATa.