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Phylogenetic (18S rRNA) analysis of this strain revealed that the isolate belonged to Thraustochytrium sp. and was labeled T01.
Analysis of this strain on TSA containing X-Gal revealed no blue coloration, even after incubation of up to 1 week.
Genome sequencing (WGS) and analysis of this strain identified a number of other putative virulence factors whose function was supported by comparative genome hybridization and expression profiling of the bacterium in hamster liver in vivo[22].
Though the intensity of Lrp5 staining was much lower in C57Bl6 MECs, analysis of this strain also showed that only basal cells expressed Lrp5, and that this staining was absent in the corresponding C57Bl6 Lrp5 −/− MECs (Figure S2).
However, although Southern analysis of this strain and RT-PCR analysis were consistent with successful insertion and expression of the resulting mRNA, it was not possible to detect protein by western blot using antisera against the epitope tag, even after attempts to enrich by immunoprecipitation (unpublished observations).
Detailed functional and structural analysis of this strain will be described elsewhere.
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We chose Synechocystis sp. PCC 6803 as a model organism and validated the results of our bioinformatic analysis for this strain with a transcript analysis.
Analysis of this adapted strain, DJO10A-JH1, shows the deletion extended over the full lantibiotic region very similar to strain NCC2705 (Fig. 6A).
A subsequent and more detailed analysis of this mutant strain, however, failed to confirm the increased SOS induction {Boonsombat, 2006 1223 /id}.
Moreover, the 454 technology and microarray assay proved to be complementary for analysis of this new strain.
PB performed the necropsy of the first pig affected by brucellosis and contributed to the isolation and analysis of this Brucella strain.
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