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Sequence analysis of the TK coding region from all five plaque isolates identified a G nucleotide insertion in the homopolymeric hot-spot G7 tract at nucleotide 435, a common mutation present in other ACVr HSV isolates [ 18, 19].
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Phylogenetic analysis of the OPV tk gene sequences revealed that they clustered with the tk gene of cowpox virus No.H1 and cowpox virus GRI (30 ) but separated from that of cowpox virus isolates from Sweden (Swe.H1and Swe.H2) and from CPXV-BR.
FS, SAR, ER, AM, CH, BM and TK undertook the analysis of the data and TK, BM, FS, SAR and ER drafted the manuscript.
GRC conceived and conducted the experiment, analyzed all of the data and wrote the body of the paper; SVS digested the proteins and collected and analyzed the proteomics data; DWH assisted in running the growth experiment and the analysis of the data; DP and TK assisted in the statistical analyses; PAH supported and assisted in the data analysis.
The analysis of tk, vgf and ha sequences from MARV isolates displayed perfect homology not only in their respective ORFs but also in the entire nucleotide sequences analyzed.
Mutational analysis of EGFR TK domain (exons from 18 to 21) and immunohistochemistry for EGFR were performed on tumour tissues derived from radical prostatectomy from 100 PC patients.
TK carried out the statistical analysis of the data.
TK participated in algorithm development, experiments & analysis of the result and writing of the paper.
TK, TDB, BS, EA and PD participated in the analysis of the study samples and interpretation of the data.
An evolutionary trace analysis of TK domain and VFTM of VKR was performed after aligning sequences of each domain, including those from insects and other invertebrates (S. mansoni and S. purpuratus).
Sequence and phylogenetic analysis of TK and IL8 gene showed that there was a very close relationship between field and vaccine strain.
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