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Analysis of the segregation of the two mtDNAs across subsequent maternal generations revealed that proportion of NZB mtDNA was preferentially reduced.
The homozygous transgenic plants were confirmed by genetic analysis of the segregation ratio of later generations.
The homozygous transgenic plants were confirmed using genetic analysis of the segregation ratio of later generations.
Statistical analysis of the segregation ratios demonstrated that the observed ratios did not differ between the studies (p > 0.05).
The basic approach consists of the analysis of the segregation of multiallelic markers, called microsatellites, which are more informative variants than SNPs, and are distributed all over the genome.
Analysis of the segregation of these variants in a reciprocal F1 cross, as well as genotyping two doubled haploids and their diploid parents, reveals both allelic (segregating) and widespread paralogous (fixed) sequence differences.
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We quantitated their chromosome loss rates with three distinct assays: analysis of the mitotic segregation of GFP-marked chromosome 2; colony-sectoring analysis of the rate of loss of the mini-chromosome Ch16; and analysis of lagging chromosomes in anaphase cells.
In contrast, top-down approaches are based on the analysis of the co-segregation of genotype and phenotype.
These grapes are the parents of two F1 populations available at the University of Udine [ 23], which were used for the analysis of segregation of the final set of markers (see below).
To assess the clinical relevance of unclassified variants, a number of approaches have been described and the analysis of segregation of the variant in affected family members has been shown to be very powerful [ 3].
The locus containing the gene that codes for flavonoid 3'-hydroxylase was identified by linkage analysis following the segregation of the SSCP of two co-segregating bands amplified from the parent 'Cabernet Sauvignon' by means of the primer pair F3int (Table 2).
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