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IRS analysis of the PR status paralleled that of MIF with high PR concentrations correlating with increased OS and RFS rates (p < 0.001 and p = 0.01, respectively).
This was demonstrated for the analysis of the PR gene.
Densitometric analysis of the PR protein signals was performed with the Phoretix 2D Expression software, v. 2005 (NonLinear USA, Durham NC, USA), after scanning the immunoblots with an Amersham Image Scanner digitalizer (GE Healthcare, Baie d'Urfé QC, Canada).
Phylogenetic analysis of the PR gene sequence showed a close phylogenetic relationship with the HIV-1 MaL strecombinantombinant virus that contains portions of subtypes A and D and an unclassifiable region that was identified in 1985 in Zaire (16 ).
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The histological classification of the HBCx-17 as a triple-negative tumour was confirmed by quantitative RT PCR analysis of the ER, PR, and ERBB2 receptors (Table 3).
In order to validate our proteomics data, western blot analysis of PR-1, PR-2, and PR-3 proteins was conducted with three biological replicates of AWF samples.
KJ provided the TMAs, performed the manual analysis of the ER and PR, and drafted the manuscript.
An analysis of the ER and PR co-expression by age group and Recurrence Score risk group is shown in Fig. 7.
In either case, this observation corroborates the sensitivity and usefulness of this assay, not only for the analysis of HIV PR activity, but also for the study and characterization of other PR variants, wild-type or mutant, and of other proteases in general, viral as well as cellular.
More recently, the 2011 St Gallen International Breast Cancer Conference [ 7] suggested using immunohistochemistry for molecular subtyping [ 5, 7, 8, 14] with the analysis of ER, PR, HER2, and Ki67 to subdivide patients into luminal A and B, HER2 positive nonluminal, and triple negative.
Few studies provided a detailed quantitative analysis of the components of the PR (i.e., fibrosis, necrosis and acellular mucin).
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