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Fig. 1 Characterization and phenotypic analysis of the mutants (KO). a OsINV3 gene in 5′-3' direction with the Tos17 insertion in the second exon in reverse orientation.
In this study, we identified the evolutionary expansion of the rice OsOPT family and showed function diversification of the duplicated genes by expression analysis and primary analysis of the mutants.
The HPLC analysis of the mutants and WT is shown in Additional file 3: Online Resource 3. Fig. 5 Overexpression of the individual ctcH and ctcJ genes and the simultaneous overexpression of ctcH and ctcJ.
Mutation testing is usually performed in four steps [41]: (1) execution of the original program, (2) generation of mutants, (3) execution of the mutants and (4) analysis of the mutants.
DNA sequence analysis of the mutants revealed between three and seven point mutations and biochemical analysis combined with STD-NMR experiments indicated that distinct molecular mechanisms were active among the three mutants.
Experimental analysis of the mutants shows that the C terminus of the molecule (comprising the last and edge β-strand) is the major contributor to amyloid fibril formation, in good agreement with theoretical predictions.
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Whole-genome gene expression analysis of the mutant revealed 12 down-regulated genes within the mapped interval.
Analysis of the mutant fitness distributions with various mixing ratios is also useful to explore local fitness landscapes.
A genetic analysis of the mutant gene, mgl-1 tm1811), mgl-1 tm1811at suggestedn delayed thatonstarvationgenesis andelayedogenesis via MGL-1.
Crystallographic analysis of the mutant proteins showed that neither had any alteration in protein structure other than the expected changes at the mutation sites.
Kinetic analysis of the mutant enzymes showed that the higher catalytic efficiency of the D77I and Y80F mutants toward α-naphthylbutyrate (C4) and α-naphthylcaprylate (C8), respectively, was due to a lower Km value.
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