For the analysis of the intramitochondrial localisation of dlps, mitochondria were isolated from cauliflower inflorescences, hypotonically lyzed, sonicated and fractionated into submitochondrial fractions.
Overexpressed Bcl-G was present throughout the cytoplasm, consistent with with a previous analysis of the subcellular localisation of overexpressed GFP-tagged hBCL-GL in HeLa cells.
Analysis of the subnuclear localisation of HP1β in differentiated C3H10T1/2 cells showed that it is not visibly delocalised from heterochromatin (data not shown).
Indeed, careful analysis of the tissue localisation of Ang-2 expression in cancer entities and tumour models other than CRC has called into question the tumour cell origin of Ang-2 (Zhang et al, 2003).
Hence, RelA appears to play a role in the progression of prostate cancer but little is known about the status of other NF- κB family members, and no simultaneous analysis of the subcellular localisation of these subunits has been previously reported in prostate tissues.
In ESCC cell lines (TE2, TE13) Tanaka et al (2010), in their analysis of the intracellular localisation of CDDP by using in-air micro-particle induced X-ray emission, recently reported that TE2 cells, which express lower MRP2 than TE13, had higher intracellular CDDP concentrations and sensitivity than TE13 cells.
Analysis of the in vivo localisation of ROS via NBT and DAB revealed that SbSOS1-overexpressing lines maintained less ROS in response to salt treatment, which indicates a reduced potential for oxidative damage resulting from salt stress.
These data provide the first comprehensive and quantitative analysis of the expression and localisation of MMPs and their inhibitors in human prostate cancer, leading to the identification of several genes involved in proteolysis as potential prognostic indicators, in particular hepsin, MTSP1, MMP26, PAI1, uPAR, MMP15, TIMP3, TIMP4, maspin and RECK.
