KT and PDB were together responsible for the idea, management and analysis of data and KT prepared the final draft of the manuscript.
A quantitative analysis of KTs with calcium-stable KT fibres (Figure 5A, graph) demonstrated that EGFP-Hec1 expression led to a clear increase in calcium-resistant side-on attachments in bipolar prometaphases with alignment defects (46.4±6.2% compared with 21±1.3% in vector prometaphase cells), indicating a longer permanence of these interactions during prometaphase in EGFP-Hec1 mitoses.
Gonads are the main sources of circulating sex hormones (Kime, 1993) therefore the analysis of 11-KT and E2 in gonads is applicable when plasma collection is not possible or the plasma volume is low (Becker et al., 1992).
The gradient used was 0−14.9 min, from 20%to80%0% B; 14.9−15.0 min, from 80%to100%0% B; 15.0−25.0 min, 100% B. For the analysis of plasma 11-KT, 20 μL of plasma was extracted(25) and the androgen was quantified by radioimmunoassay.
The accuracy and precision of proposed methods are determined by intraday and interday analysis of KT at three different concentration levels within optimized linearity range, and each concentration was replicated five times and presented in Table 2.
KT and CF carried out the survey, participated in the analysis of the data, and helped to draft the manuscript.
NM, KT, and ZI provided peripheral blood samples and clinical information, and performed analysis of the data.
Parametric study was followed by a set of the nonlinear regression analyses to develop SCF parametric equations for the fatigue analysis of ring-stiffened KT-joints under IPB loadings.
Monopolar spindles cannot form bioriented KT-MT attachments, and they present kinetochores in a floret-like configuration that allows easy analysis of individual KT-MT attachments.
Kinetic analysis of the results indicates that the dissolution of limestone is according to the shrinking core model with surface control, i.e. 1−(1−3)1/3 = kt.
The kinetic analysis of the release data was done using Korsmeyer and Peppas equation or the Power law equation (Peppas 1985): M t / M ∞ = kt n (3).
