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The isolate was identified based on morphology and sequence (Genbank Accession No. JQ284030) analysis of the ITS region of the rDNA.
The results of the neighbour-joining analysis of the ITS region sequences of the 17 Aspergillus strains and VP4 are shown in the dendrogram of Fig. 1.
The maximum likelihood analysis of the ITS sequence data indicates that this procedure can be used to differentiate A. aotearoa from the other species of Armillaria present in New Zealand, including A. hinnulea which is difficult to distinguish morphologically.
Analysis of the ITS sequences of the Taiwanese isolates of P. noxius revealed that the 5.8 S region was identical in all isolates, while significant sequence variation was observed in ITS regions.
Sequence analysis of the ITS rDNA region indicated 99% similarity with GenBank sequences of G. castanea confirming the identity of this species characterized as the main causal agent of rot of the ripe nuts, both before and after picking [2].
After screening yeast strains with Sudan III, morphological and physiological characterization and identification of such potential oleaginous yeasts, they were further identified by sequence analysis of the ITS and the D1/D2 domains of the 26S rRNA gene and the sequences obtained from this study and that of closely related sequences from GenBank are displayed in Fig. 2.
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MP analysis of the ITS-1 sequences (positions 1592 2454, 169 informative characters, ti/tv ratio 0.6000 7.0000) gave 32 trees, one of which was retained after successive weighting.
Using single-strand conformation polymorphism (SSCP) and selective sequence analysis of the ITS-2, genetic variation in the nematode was found [ 10], with nucleotide sequence variability mainly in loops or bulges of the predicted secondary structure.
In addition to the mathematical analysis of the algorithm, its effectiveness is supported through simulation.
Other information, including dates of NRC's interactions with Duke Energy, its own analysis of the threat, and its assertion that the threat was credible, had no clear security component, nuclear safety advocates said.
In particular, it should include an analysis of the impacts on its oil and gas reserves.
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