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A comprehensive analysis of the inserts confirmed by tests on laboratory stands, showed the interaction between the thermal fatigue and abrasive wear, combined with the process of oxidation.
Sequence analysis of the inserts revealed that these represented NF1 transcripts with specific insertions/deletions.
Sequencing analysis of the inserts matched exactly with the targeted gene sequences in the database (website: http://www.ncbi.nlm.nh.gov/blast).nh.gov/blast
According to the analysis of the inserts, sucrose was found in 47.5% of the medicines, and the antiemetics drug class was the only one devoid of sugar products (Table 4).
BLAST similarity analysis of the inserts of the cDNA clones sequenced against the mRNA sequences of the NCBI RefSeq (release 49) revealed that the cDNA sequences corresponded to 11,298 human genes and more than 10,000 genes in each of mice, cattle, and dogs (Table 5).
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Analysis of the insert sites in the P. berghei genome showed that insertion occurred exclusively in the expected TTAA insertion site.
"Protein engineering" analysis of the inserted region shows that it is folded in the transition state.
Analysis of the insert DNA sequence as described above, revealed an ORF (1890 bp) with ATG as start codon and TAG as termination codon.
Colonies were picked and plasmids isolated by standard miniprep following restriction analysis of the insert.
Further analysis of the insert sequences identified through the different genetic selections did not reveal the presence of common "seed" regions or extended sequence similarity or complementarity with previously reported human miRNAs.
Analysis of the insert size distribution showed that the overlapping ditags tended to be small fragments.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com